Identification of pathogens and virulence profile of Rhodococcus equi and Escherichia coli strains obtained from sand of parks

The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic classification showed that 63 E. coli isolates belonged to groups B1 (52.4%), A (25.4%) and B2 (22.2%). No E. coli serotype O157:H7 was identified. Eggs of Toxocara sp. were found in three parks and genetic material of bovine Coronavirus was identified in one sample of one park. No Salmonella spp. and Rotavirus isolates were identified in the samples of sand. The presence of R. equi, Toxocara sp, bovine Coronavirus and virulent E. coli isolates in the environment of parks indicates that the sanitary conditions of the sand should be improved in order to reduce the risks of fecal transmission of pathogens of zoonotic potential to humans in these places.

Mastite bovina por Pasteurella multocida: estudo de nove casos / Bovine mastitis by Pasteurella multocida: a study of nine cases

The uncommon occurrence of bovine mastitis by Pasteurella multocida is reported in nine cows. Mammary pasteurellosis was predominantly diagnosed predominantly in clinical cases, in dairy cows milked in the presence of calves. Low interference of immunosuppressive or predispose factors and absence of pulmonary signs were observed in animals. In vitro multiple drug resistance of isolates was observed to three or more conventional antimicrobials, especially with use of tetracycline and gentamicin. Epidemiologic findings, clinical signs, and antimicrobial profile were discussed in nine cases of bovine mastitis caused by P. multocida.

Surto de mastite bovina causada por linhagens de Pseudomonas aeruginosa multirresistentes aos antimicrobianos / Outbreak of bovine mastitis caused by multiple drug resistant Pseudomonas aeruginosa strains

Uncommon outbreak of mastitis caused by Pseudomonas aeruginosa strains in 19 cows was reported.Enrofloxacin (73.7%) and danofloxacin were in vitro effective against those strains. In vitro multipledrug resistance of the isolated bacteria was observed to five or more conventional antimicrobials usedfor mammary therapy, including ampicillin (100.0%), cefalexin (100.0%), cloxacillin (100.0%),gentamicin (100.0%), penicillin/novobiocin (100.0%), cefoperazone (52.6%), tetracycline (100.0%),florfenicol (94.7%), and neomycin (73.7%). Epidemiological findings, clinical signs, in vitrosusceptibility profile, control measures, and public health risks were discussed in cows infected by P. aeruginosa.

Peracute bovine mastitis caused by Klebsiella pneumoniae / Mastite bovina hiperaguda causada por Klebsiella pneumoniae

Relata-se a ocorrência de graves sintomas de mastite hiperaguda em vaca, causada por Klebsiella pneumoniae, na terceira semana de lactação. Descrevem-se aspectos epidemiológicos, sintomas clínicos, procedimentos de diagnóstico microbiológico, conduta terapêutica e medidas de controle.

Teste tuberculinico e vacinacao BCG intradermica em servidores de uma empresa publica. / Tuberculin test and BCG intradermic vaccination in workerrs of a Public Company

Os autores descrevem o inicio da implantacao de programa de controle da tuberculose em Empresa Publica, no Municipio de Sao Paulo, mostrando os resultados do cadastramento tuberculinico seguido de aplicacao da vacina BCG intradermica nos "reatores negativos". Foram aplicados 8.016 testes tuberculinicos e 3.637 vacinas BCG intradermicas. O indice de infeccao tuberculosa situou-se ao redor de 39%. E tambem discutida a introducao da vacina BCG intradermica no exame pre-admissional